吕丹阳,蒋磷雷,王丹凤,邓云.黑蒜黑枸杞复合饮料制备过程中酶解工艺优化[J].包装工程,2019,40(23):52-58.
LYU Dan-yang,JIANG Lin-lei,WANG Dan-feng,DENG Yun.Optimization of Enzymatic Hydrolysis Conditions for Preparation of Black Garlic-Black Medlar Compound Juice[J].Packaging Engineering,2019,40(23):52-58. |
| 黑蒜黑枸杞复合饮料制备过程中酶解工艺优化 |
| Optimization of Enzymatic Hydrolysis Conditions for Preparation of Black Garlic-Black Medlar Compound Juice |
| 投稿时间：2019-08-27 修订日期：2019-12-10 |
| DOI：10.19554/j.cnki.1001-3563.2019.23.008 |
| 中文关键词: 黑蒜 黑枸杞 酶解 复合饮料 模糊评价 |
| 英文关键词: black garlic black medlar enzymatic hydrolysate compound juice fuzzy synthetic evaluation |
| 基金项目:云南省科技厅重大科技专项（2018ZI001）；上海市科委项目（19DZ2284200） |
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| 中文摘要: |
| 目的 以黑蒜、黑枸杞等为主要原料，以出汁率、透光率、饮料中花色苷含量和DPPH自由基清除率为依据，研究酶解条件对复合饮料产率和稳定性的影响。方法 选择纤维素与果胶酶比例、复合酶添加量、酶解温度和酶解时间为自变量的4因素3水平的正交实验优化酶的最佳酶解条件。结果 各因素对复合饮料的模糊综合评分值的影响主次顺序为复合酶添加量>酶解温度>酶解时间>复合酶比例；最佳酶解工艺条件，复合酶质量分数 0.06 %，纤维素酶与果胶酶的质量比为2︰1，酶解温度为50 ℃，酶解时间为3 h。 在此优化工艺条件下，出汁率为91.04 %，透光率为77.27 %，饮料中花色苷的质量浓度为167.11 mg/L，DPPH自由基清除率为58.81 %。结论 该工艺生产的复合饮料出汁率高，抗氧化活性强。 |
| 英文摘要: |
| The paper aims to research the effect of enzymolysis condition on yield rate and stability of compound juice based on juice yield, light transmittance, total anthocyanin content and DPPH free radical scavenging rate with black garlic and black medlar as the main ingredients. Ratio of cellulose to pectinase, enzyme concentration, hydrolysis temperature and hydrolysis time were selected as the optimal conditions for enzymatic hydrolysis for orthogonal experiment of 4 factors and 3 levels. The results showed that the order of influence on the indexes of the compound juice was enzyme concentration > hydrolysis temperature > reaction time > enzyme ratio. The optimal conditions for enzymatic hydrolysis were cellulase: pectinase mass ratio 2︰1, compound enzyme mass fraction of 0.06%, enzymatic hydrolysis temperature of 50 ℃, and enzymatic hydrolysis time of 3 h. Under this optimized process condition, the juice yield was 91.04%, the light transmittance was 77.27%, the anthocyanin content in the juice was 167.11 mg/L and the DPPH free radical scavenging rate was 58.81%. The compound juice produced by this process has high juice yield rate and high antioxidant activity. |
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