赵梅慧,庞林江,成纪予,陆国权,路兴花,王孙杰.长喙壳菌侵染对不同抗性甘薯细胞壁的影响[J].包装工程,2022,43(21):10-16.
ZHAO Mei-hui,PANG Lin-jiang,CHENG Ji-yu,LU Guo-quan,LU Xing-hua,WANG Sun-jie.Effects of Ceratocystis Fimbriata Infection on Cell Wall of Different Resistant Sweet Potato[J].Packaging Engineering,2022,43(21):10-16. |
| 长喙壳菌侵染对不同抗性甘薯细胞壁的影响 |
| Effects of Ceratocystis Fimbriata Infection on Cell Wall of Different Resistant Sweet Potato |
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| DOI：10.19554/j.cnki.1001-3563.2022.21.002 |
| 中文关键词: 甘薯 黑斑病 长喙壳菌 细胞壁 |
| 英文关键词: sweet potato black rot Ceratocystis fimbriata cell wall |
| 基金项目:国家甘薯产业技术体系项目(CARS–10);浙江省自然科学基金(LY19C200015) |
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| 中文摘要: |
| 目的 研究不同黑斑病抗性品种甘薯的细胞壁变化特点，探索与抗性相关的特征。方法 以抗性不同的鲜食优质甘薯品种“心香”和“烟薯25”作为供试材料，通过接种长喙壳菌培养，分析2个品种对黑斑病的抗性特征，通过测定贮藏期间细胞壁相关指标，分析其可能影响抗性的机理。结果 接种长喙壳菌后，“烟薯25”黑斑病病斑表面直径、内部直径、综合直径、深度和腐烂指数均小于“心香”，综合表现抗性较好。染菌甘薯病变部位呈现出硬度、原果胶含量逐渐降低，可溶性果胶、木质素含量逐渐增加，纤维素含量先增加后降低，纤维素酶先降低后增加，β–葡萄糖苷酶呈逐渐增加趋势。“烟薯25”的病变组织硬度，可溶性果胶、纤维素、木质素含量和β–葡萄糖苷酶活性显著高于“心香”的，但原果胶含量和纤维素酶活性显著降低，染菌后两品种间差异更大。结论 长喙壳菌侵染甘薯会导致病变部位硬度降低，细胞壁结构解体。“烟薯25”对黑斑病抗性表现优于“心香”，其细胞壁结构组成较为完整且硬度较高。 |
| 英文摘要: |
| The work aims to explore the relevant characteristics of resistance to black rot by studying the cell wall changes of sweet potatoes with different black spot resistance. Fresh sweet potato varieties 'Xinxiang' and 'Yanshu 25' with different resistance were used as test materials. The resistance characteristics of the two varieties to black rot were analyzed by inoculating the Ceratocystis fimbriata culture method. The possible mechanism of resistance was analyzed by determining relevant indexes of the cell wall during storage. After inoculation with Ceratocystis fimbriata, the surface diameter, internal diameter, comprehensive diameter, depth, and decay index of 'Yanshu 25' with black rot were less than those of 'Xinxiang', and its comprehensive resistance was better. The diseased region of infected sweet potato showed a gradual decrease in hardness and protopectin content, a gradual increase in soluble pectin and lignin content, a first increase and then a decrease in cellulose content, a first decrease and then an increase in cellulase, and a gradual increase in β-glucosidase content. Hardness, soluble pectin, cellulose, lignin content and β-Glucosidase activity of diseased tissue of 'Yanshu 25' were significantly higher than those of 'Xinxiang'. While its content of protopectin and cellulase activity decreased significantly. The difference between these two varieties was greater after infection. The infection of sweet potato by Ceratocystis fimbriata will lead to the decrease of hardness and the disintegration of the cell wall structure. The resistance of 'Yanshu 25' to black spot was better than that of 'Xinxiang'. Its cell wall structure is more integrated, and the hardness is higher. |
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