文章摘要
崔方超,周闪闪,王当丰,檀茜倩,李秋莹,李婷婷,励建荣.氨基功能化抗菌碳量子点的制备及抗菌性能研究[J].包装工程,2023,44(5):121-129.
CUI Fang-chao,ZHOU Shan-shan,WANG Dang-feng,TAN Xi-qian,LI qiu-ying,LI Ting-ting,LI Jian-rong.Preparation and Antibacterial Properties of Amino Functionalized Antibacterial Carbon Quantum Dots[J].Packaging Engineering,2023,44(5):121-129.
氨基功能化抗菌碳量子点的制备及抗菌性能研究
Preparation and Antibacterial Properties of Amino Functionalized Antibacterial Carbon Quantum Dots
  
DOI:10.19554/j.cnki.1001-3563.2023.05.016
中文关键词: 碳量子点  热解法  食源性致病菌  抗菌  抗菌机制
英文关键词: carbon quantum dots  pyrolysis method  foodborne pathogenic bacteria  antibacterial  antibacterial mechanism
基金项目:国家重点研发计划课题(2019YFD0901702)
作者单位
崔方超 渤海大学 食品科学与工程学院 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁 锦州 121013 
周闪闪 渤海大学 食品科学与工程学院 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁 锦州 121013 
王当丰 渤海大学 食品科学与工程学院 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁 锦州 121013 
檀茜倩 渤海大学 食品科学与工程学院 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁 锦州 121013 
李秋莹 渤海大学 食品科学与工程学院 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁 锦州 121013 
李婷婷 大连民族大学 生命科学学院辽宁 大连 116600 
励建荣 渤海大学 食品科学与工程学院 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁 锦州 121013 
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中文摘要:
      目的 以精胺、多巴胺为原材料合成氨基功能化抗菌碳量子点,为进一步将其应用于食源性致病菌消除领域提供参考。方法 利用精胺、多巴胺通过热解法合成精胺碳点、多巴胺碳点和精胺/多巴胺碳点(SPM–CDs、DA–CDs、SPM/DA–CDs),通过透射扫描电镜,X射线光电子能谱、红外光谱、Zeta电位、紫外光谱和荧光光谱对碳点进行表征,选取食源性致病菌金黄色葡萄球菌和大肠杆菌作为供试菌株,采用微量肉汤稀释法研究碳点及前体物的抗菌性能。结果 SPM–CDs、DA–CDs、SPM/DA–CDs的分散性好,平均粒径分别为(4.25±0.89)、(3.90±0.67)、(4.0±0.96)nm;在365 nm紫外灯照射下3种碳点均能发出荧光,表面都带有较高的正电荷并且含有C=C、C−O、O−H等化学键;抗菌实验表明,SPM/DA–CDs对金黄色葡萄球菌和大肠杆菌抑制效果显著,最小抑菌质量浓度分别为0.25 mg/mL和0.5 mg/mL,而SPM–CDs、DA–CDs和前体物对2种菌的抑制效果较差;SEM结果表明碳点能造成细菌表面凹陷、破裂,从而导致细菌死亡。结论 本研究合成的氨基功能化抗菌碳点具有优异的抗菌效果,有望应用于食源性致病菌的防治领域,降低食品中致病菌带来的安全隐患,保障食品安全和人类身体健康。
英文摘要:
      The work aims to synthesize amino-functionalized antibacterial carbon quantum dots with spermine and dopamine as raw materials, to provide reference for their further application in elimination of foodborne pathogens. Spermine carbon dots, dopamine carbon dots and spermine/dopamine carbon dots (SPM–CDs, DA–CDs, SPM/DA–CDs) were synthesized by pyrolysis of spermine and dopamine. The carbon dots were characterized by transmission scanning electron microscopy, X-ray photoelectron spectroscopy, infrared spectroscopy, zeta potential, ultraviolet spectroscopy and fluorescence spectroscopy. Foodborne pathogenic bacteria Staphylococcus aureus and Escherichia coli were selected as the test strains. The antibacterial properties of carbon dots and their precursors were studied by the micro broth dilution method. The results showed that SPM-CDs, DA-CDs and SPM/DA-CDs had good dispersion, with average particle sizes of (4.25±0.89), (3.90±0.67) and (4.0±0.96) nm, respectively. Under the irradiation of 365 nm UV lamp, the three carbon spots could emit fluorescence, and the surface had high positive charge and contained chemical bonds such as C=C, C−O, O−H; The antibacterial experiment showed that SPM/DA-CDs had significant inhibitory effect on Staphylococcus aureus and Escherichia coli, and the minimum inhibitory concentration was 0.25 mg/mL and 0.5 mg/mL respectively, while SPM-CDs, DA-CDs and precursors had poor inhibitory effect on the two bacteria; SEM results showed that the carbon spots could cause the bacterial surface depression and rupture, leading to the death of bacteria. The amino functionalized antibacterial carbon dots synthesized in this work have superior antibacterial effect and are expected to be applied to the prevention and treatment of foodborne pathogens, reduce the potential safety hazards caused by pathogenic bacteria in food, and ensure food safety and human health.
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