Automotive human-computer interaction system is complex and dynamic. With the increasing popularity of the in-vehicle electronic information system, massive intensive multi-dimension real-time information has been implanted in the system. To ensure the driver focus the unconscious cognition on the primary driving task in the complex and dynamic interaction system, human unconscious cognition can be used to guide the design of in-vehicle information system user interface and optimize the interface layout of the vehicle electronic instrument panel in the car driving seat. The design guidelines are based on the combination of human unconscious cognition and behavior pattern. The design guidelines summarize the principles of interface layout design of vehicle electronic instrument cluster, put forward the optimization scheme of core visual information layout design, and verify the usability of the design scheme through user survey data and reliability test.By following the guidelines of user’s unconscious cognition pattern, we can improve the design of in-vehicle electronic cluster which conforms to the user’s unconscious cognition and needs.