Taking the regional culture of Shikumen as the research target, the paper aims to explore the innovative ways in witch regional cultural characteristics applied to the design of wayfinding system. Shikumen sample pictures are coolected and R language cluster analysis is used to select typical samples; semantic difference method is used to obtain Shikumen style image; analytic hierarchy process is used to construct Shikumen design factor extraction model and judgment matrix, design factors are extracted from three aspects of shape, color and material, each factor is calculated for importance and applied to wayfinding system; through the evaluation of style image, the wayfinding system scheme is selected. The style image and design factors of Shikumen are obtained, and applied to the design of wayfinding system of the Cool Dock. By extracting the style image and design factors of regional culture, people can enhance the integration of the wayfinding system and regional culture, which can not only provide power for the innovative design of wayfinding system, but also can create a better cultural atmosphere for the place and guide the direction of cultural inheritance.