Based on the perspective of authenticity protection of Dong culture, this paper traces the origin of Dong traditional cultural elements and constructs a meme database to explore the objective process of authenticity design of intangible cultural heritage visual elements. The paper uses semiotics to guide the collection and extraction of data, applies memetics to analyze and reorganize symbols to realize the digital extraction and atlas representation of the characteristics of Dong traditional elements, and then uses fuzzy analytic hierarchy process (F-AHP) to judge the matrix weighting of Dong symbols. By constructing a complete evaluation system of meme map of dong traditional elements and its follow-up application process, the traditional elements are effectively applied to the design of guide system to realize the authenticity and locality design of traditional elements and form a design mode based on the deep thinking of innovative design of Dong culture. In conclusion, the design of the guide system guided by the Dong settlement culture meme makes the guide system not only realize its own functionality, but also greatly enhance the cultural added value, expand the transmission path and inheritance value of the Dong culture, and enhance the authenticity protection of the Dong settlement culture.