Scan is not only the common functional interface of many mobile applications, but also the entrance of more expanded functions. The work aims to study the factors affecting the usability of scan interface and provide theoretical support and design guidance for design of scan interface. The existing scan interfaces were summarized and analyzed. The interface composition was summarized into three kinds of component:scan box, mode switching icon, and functional auxiliary icon. With layout relation between components and isolation of icons and background as independent variables, an icon search experiment under different levels of independent variables was designed, and the data in the search process were recorded by eye tracking. The effects of independent variables on the usability of scanning interface were studied by comparing three kinds of eye movement data, namely response time, number of fixation points and saccadic times, through repeated measurement of ANOVA. The experimental data showed that there were significant differences among the three kinds of data in terms of component layout relation, and the eye movement data of the adjacent component layout relation was superior to that of the distance type. There were significant differences in response time and number of fixation points in isolated icons and background, and the eye movement data of icon background isolation is superior to that of non-isolated icons and background. Adjacent component layout and isolated icons and background have a positive impact on the usability of the scan interface.