The work aims to study the effect of color environment in sleeping capsule on crew emotion under different emotional stimuli. 30 subjects (15 men and 15 women) were respectively induced by neutral and negative VR emotional films, and then experienced the color environment of the simulated sleeping capsule by VR technology to complete the subjective emotional evaluation. 20 channel EEG signals of subjects experiencing the color environment were collected. Variance analysis was carried out on subjective emotion evaluation, and EEG signals were preprocessed to analyze the EEG signal energy of α and β frequency bands. Subjective emotional evaluation showed that:in terms of color environment comfort, different color environments (cold color, warm color, colorless) showed significant differences (p=0.000<0.05); and in terms of emotional relief, different color environments (cold color, warm color, colorless) showed significant differences (p=0.000<0.05). EEG signal displayed:The average power ratio of left and right lobe of inferior frontal lobe and parietal lobe under α wave was the lowest in cold color environment, and the highest in warm color environment. The average power ratio of left lobe and right lobe in inferior frontal lobe under β wave was the lowest in cold color environment and the highest in warm color environment. In the narrow and closed simulated spacecraft sleeping capsule environment, the emotional regulation of the color environment on the subjects can be reflected in their physiological state and psychological feelings, and their subjective feelings and objective physiological data analysis results can be fitted to a certain extent. Among them, the subjective and objective values of blue environment, green environment and white environment are the best, which can effectively improve and form a good emotional state. Finally, the feasibility and reliability of the optimization strategy for the color design of the spacecraft sleeping capsule are verified through the simulation of the color design practice of the spacecraft sleeping capsule.