The work aims to propose an interface layout optimization strategy based on attention allocation to solve the problem that the interface layout design of existing children's education apps lacks relevance. The eye-movement indicators were collected from 20 subjects during the activity of browsing the application interface through an eye-movement experiment. A total of three eye-movement indicators, namely total gaze duration, first gaze duration and first gaze duration, were extracted for each region, and the subjects' attention allocation and the influencing factors were statistically analyzed. The results showed that children's attention was the most concentrated in the middle area of the upper half of the screen; that colour and background had an effect on children's attention but did not cause contrasting results; and that there was a negative correlation between total gaze duration and first gaze time. In addition, the subjects' level of mobile device operation was assessed and analyzed and no correlation was found between their level of operation and attention allocation. The practical case shows that the interface layout optimization based on children's attention distribution can achieve the purpose of optimizing the interface layout, so that important information or content has a higher chance of being read. It can also maximize the delivery of information and improve the efficiency of information reception, thus providing a reference for the optimization of the interface layout of children's educational applications.