The work aims to analyze, check and improve the design scheme of subway car handrails in combination with the man-machine simulation analysis software Jack to satisfy the convenience and comfort of subway passengers. According to the main technical specifications of Type A cars in GB/T 7928—2003 and the size requirements of passenger car handrails in GB 13094—2017, a 3D model of subway cars was built in CAD. According to GB 10000—1988, the human body digital models of the 5th percentile adult female and 95th percentile adult male were established in Jack. Rapid upper limb assessment and comfort assessment were carried out to obtain the overall comfort score of standing posture. Through ergonomic analysis results, the number layout and height setting of handrails in subway cars were designed and improved, and the comfort level of passengers' standing posture was verified again by Jack. The results show that the improved design scheme meets the convenience and comfort requirements of passengers, and provides a reference for the ergonomic design of the handrails of subway cars. The related design parameters can supplement the existing standards.