The work aims to put forward an immersive experience and interaction design method of intangible cultural heritage performance based on biofeedback by exploring and analyzing the immersive experience of intangible cultural heritage performance, the digital form characteristics, and the evolution of the relationship between viewing and performance. From the real environment to the virtual space development process, the audience's viewing mode and interaction mode were studied, the human-computer interaction mode of multi-person collaboration was constructed, and two universal immersive experience design methods of audience performance avatar design and multi-person co-creation interactive narrative were proposed, which were then practiced and elaborated through performance examples. Through the actual experience of the audience, the effect of natural interaction and cultural transmission in the immersive experience of intangible cultural heritage performance is enhanced, and a new way is opened for the protection and inheritance of intangible cultural heritage performance. The research provides feasible suggestions for future immersive experience of intangible cultural heritage.