The work aims to analyze the symbolic features of Miao braid embroidery patterns in southeast Guizhou based on the Morris semiotic triplicate method, explore the application methods and transformation paths of ethnic embroidery patterns in modern cultural and creative design, and provide more ideas and references for the interpretation and research of Miao braid embroidery patterns in southeast Guizhou from the perspective of semiotics. Through field investigation, literature collection, design practice and other methods in Miao's residential area in southeast Guizhou, the representative symbol features of the process, decoration, color and other aspects of Miao braid embroidery patterns in southeast Guizhou were discussed and analyzed, and the symbolic relationship between Miao braid embroidery patterns in southeast Guizhou was interpreted from the aspects of texturing, pragmatically and semiotically with the Morris semiotic trism. With modern design practice as the method, design factors were extracted from representative symbolic elements. Cultural and creative products were used as the carrier for design transformation. Miao braid embroidery in southeast Guizhou has distinct artistic features and certain regional characteristics and aesthetic trends. The semiotic elements of process, ornamentation and color are extracted and derived from the dimensions of language structure, pragmatics and semantics, so as to explore multiple possibilities of transformation and innovative application of design elements of Miao braid embroidery in southeast Guizhou, which can not only make modern design products contain ethnic cultural characteristics, but also contribute to the inheritance and development of Miao braid embroidery patterns in southeast Guizhou.