The work aims to construct a visual science popularization map of the cultural gene of the Great Wall at Jiumenkou based on the concept of cultural genes. Taking the Jiumenkou Great Wall, a world cultural heritage, as the research object, the cultural resources of the Great Wall as well as its surrounding areas were deeply explored through literature review and field investigation. Based on different expression forms and identifying dimensions of cultural genes, the cultural resources of the Great Wall at Jiumenkou were divided into dominant cultural genes and recessive cultural genes. The core cultural genes were extracted and the internal cultural logic structure was effectively organized. And on this basis, the cultural gene pedigree map of the Great Wall at Jiumenkou was constructed. The visual rhetoric method was used for symbol translation, and the grid design system was used for visual hierarchy division and information visualization design. The concept of cultural is introduced from the perspective of design, and historical and cultural data is translated into visual language that familiar to the audience. The science popularization map of the Great Wall at Jiumenkou presents the cultural information related to the Great Wall in a scientific and systematic way, with a view to providing reference for the development and inheritance of the Great Wall culture and helping the construction of the Great Wall National Cultural Park.