The work aims to seek reasonable subway carriage layout through design intervention and avoid bad behavior habits in public environment with the help of behavior orientation in the perspective of persuasive thinking. Firstly, the connotation of persuasive design and the mechanism of availability were sorted out, and the combined characteristics of persuasive design and availability were summarized. Secondly, the persuasive design thinking was used to discuss subway passenger behavior and its mechanism, and to seek persuasive opportunities in subway carriage space design. Finally, the main factors causing the uneven distribution of passenger flow were found out through investigation and field observation. The design framework of subway carriage space and persuasive design model of subway carriage space were constructed by analyzing the three elements of carriage space design and combining the FBM and the mechanism of supply. As a result, the persuasive design strategy of subway carriage centered on passenger behavior was proposed, namely using the differentiation of different function comfort to adjust attraction rate of area, using the internal elements transparency of the system to get path persuasion and using the symbol of the visual communication system to achieve implicit behavior triggers. The introduction of persuasive thinking in the design of subway carriage space can effectively alleviate the uneven distribution of passenger flow and provide a new idea for the improvement of subway space design.