The work aims to study the method of regional public brand design under Jiangsu regional culture. With Jiangsu regional culture connotation as a value basis, the Jiangsu regional culture characteristics, the role of regional culture in brand design and the current development status of Jiangsu regional public brands were analyzed in combination with high-quality brand design cases to extract visual elements from Jiangsu regional culture and discuss the method of regional public brand design. The brand design should follow the instruction of "constructing a systematic standard, considering regional differences, highlighting the prominent advantages and converging marketing", and set a guideline for a forward looking and general applied agricultural regional design. The regional public brand image driven by regional culture strengthens the sustainable interaction between products and consumers, guiding consumers to see the process of agricultural products from the field to the hand, promoting the formation of local representative regional public brands, and upgrading of regional cultural industries with point-to-surface radiation to help rural revitalization.