Based on the theory of cultural genes, the work aims to interpret the factors of cultural genes contained in Nanfeng Nuo masks which are in line with the cultural identification and propose the cultural gene expression with symbol as the unit of identification according to the importance of the audience's perception of factors. In order to quantitatively evaluate the culture of Nuo masks, by extracting cultural genes from Nanfeng Nuo masks, AHP was used to combine qualitative and quantitative research methods, to build a cultural genealogy map - evaluation model tree. According to the cultural gene map of Nanfeng Nuo masks, the symbol weight was derived. Through the mimetic environment theory, the symbols with brand identification were analyzed from four levels of objective environment, information selection, media processing and mimetic environment, and a substantial expression of cultural genes was derived. From the perspective of cultural genes, the combination of qualitative and quantitative research is conducive to promoting the formation of the information environment and enhancing the sense of cultural identity for the dissemination of Nanfeng Nuo culture.