The work aims to provide a comprehensive and quantifiable thinking method for packaging design of regional agricultural and sideline products by cultural ecology theory. Based on the theory of cultural ecology, the cultural ecosystem of regional agricultural and sideline products was summarized by layers. Through representative evaluation, the most representative cultural ecological level of the product was selected. The cultural content of the cultural level was analyzed and the concrete design elements were extracted. The applicability of concrete elements was evaluated, and the elements with strong cultural characteristics and high design value were selected for packaging design. With the ecological Simiao Rice in Rezhe Town, Pingyuan County, Meizhou City as an example, the corresponding design practice was carried out. Based on the theory of cultural ecology, the natural and social environments for regional agricultural and sideline products can be summarized comprehensively, and a more systematic thinking path can be provided for the packaging design of regional agricultural and sideline products.