The work aims to scientifically design the operation control interface of typical drones, to enhance the interactive control ability of operators in the context of human-machine collaboration and reduce operational errors and inefficiencies. The problems existing in the interface interaction process and the expectations of users in the human-machine collaboration context were investigated. The design scheme was evaluated through the usability test evaluation scale and eye-tracking experiments. When using the optimized scheme to complete the set tasks, operators had shorter response time and smaller average pupil diameter, took less time and got higher accuracy rate. The operators' perception, understanding and prediction ability of the optimized human-machine collaboration control interface are significantly better than those before optimization, which verifies the effectiveness of the optimized interface interaction design scheme.