The work aims to explore the iconology research and cultural and creative products development paths of the Yao festival portrait "Meishan Scroll". Firstly, data collection was conducted on contents such as "Meishan Scroll", interviews with veteran artists, questionnaires, and existing cultural and creative products of the Yao ethnic group. Through methods such as role analysis, statistics and mining of questionnaire results, and analysis of existing cultural and creative products, data analysis was conducted to determine the functions and design positioning of the cultural and creative products with the theme of "Meishan Scroll". Secondly, 54 characters were selected for two-dimensional illustration drawing and applied to the design of playing cards and emojis. Based on the principle of the connection between role responsibilities and modern social division of labor, six characters were selected for modeling, 3D printing and painting. The color settings of the characters were derived from the local natural environment and characteristic products of the Yao ethnic group, and the characters were applied to fields such as teaching aids and packaging of local specialties. A design path of the "Meishan Scroll" cultural and creative product was concluded, namely "iconology interpretation + two-dimensional and three-dimensional transformation + application". In conclusion, the design result provides a reference for the development of cultural and creative products of festival image resources, and are applied to the promotion of the "Yao Festival" brand, the introduction of traditional festival culture into the art classrooms of elementary schools and kindergartens, as well as local food packaging design, which can help to enhance the dissemination of the Yao festival culture, improve the cultural recognition of the Yao festival tourism brand, and promote the high-quality development of rural festival tourism.